A Comparative Study of Hematocrit Levels in Packed Red Cells Using Sedimentation and Centrifugation Method
Abstract
Packed Red Cells (PRC) are blood components that require appropriate processing to achieve hematocrit levels that meet quality standards. The separation method may influence the hematocrit (HCT) level of the final PRC product. This study aimed to determine and compare hematocrit levels in PRC products prepared using sedimentation and centrifugation at the Indonesian Red Cross (PMI) Blood Management Unit, Bojonegoro Regency. This quantitative comparative study included eight blood units selected by purposive sampling, with four units processed using sedimentation and four using centrifugation. Hematocrit levels were measured using the Mission HemoPro analyzer and compared using the Mann–Whitney U test. PRC prepared using sedimentation had hematocrit levels ranging from 52% to 63%, with a median of 56.0%, whereas PRC prepared using centrifugation had hematocrit levels ranging from 70% to 76%, with a median of 73.5%. Hematocrit levels differed significantly between the two groups (exact p=0.029), with higher values observed in the centrifugation group. In conclusion, PRC products prepared using centrifugation showed higher hematocrit levels than those prepared using sedimentation under the conditions of this study. Further studies with larger, statistically determined sample sizes are needed to confirm these findings.
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